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The gene expression blog

All Posts

How to bring light to PCR: TaqMan probe or SYBR Green dye?

Functional validation of a qPCR instrument

Is it better to pipet duplicates or triplicate reactions in real-time PCR?

Why is the PCR amplification efficiency still ignored?

Benchmarking RNA sequencing sensitivity using transcriptome-wide RT-qPCR data

The impact of pipetting errors on PCR amplification efficiencies

Novel splice junctions identified in RNA sequencing studies: noise or interesting biology?

Seven tips for bio-statistical analysis of gene expression data

Four tips for RT-qPCR data normalization using reference genes

How to improve qPCR assay design by understanding the impact of primer mismatches?

Embracing digital PCR in peaceful coexistence with qPCR

How to find stably expressed microRNAs

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