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How to improve qPCR assay design by understanding the impact of primer mismatches?

Jo Vandesompele - Sep 8, 2013

Designing a well working and reliable qPCR assay is a lot of work. Apart from prediction of specificity, the assay should also be screened for possible secondary DNA structures that interfere with efficient amplification (see Figure 1 in D'haene et al., Methods, 2010) and for the presence of single nucleotide polymorphisms (SNPs) that impair amplification of the variant allele.

Topics: SNP- quality control- primer

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